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Tel: 17734809689
Tel: 86-371-86259723
Tel: 17734809689
Tel: 17734809689
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Tel: +86-371- 86259723
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| Product Name: | L-Leucine |
| Synonyms: | (s)-2-amino-4-methylvalericacid;1-Leucine;2-Amino-4-methylpentanoic acid;2-amino-4-methylpentanoicacid;2-amino-4-methyl-valericaci;4-methyl-l-norvalin;4-methyl-norvalin;alpha-Amino-gamma-methylvaleric acid |
| CAS: | 61-90-5 |
| MF: | C6H13NO2 |
| MW: | 131.17 |
| EINECS: | 200-522-0 |
| Product Categories: | Food and Feed Additive;Amino Acid Derivatives;Leucine [Leu, L];Amino Acids;Amino Acids and Derivatives;alpha-Amino Acids;Biochemistry;Nutritional Supplements;L-Amino Acids;Amino Acids;amino |
| Mol File: | 61-90-5.mol |
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| L-Leucine Chemical Properties |
| mp | >300 °C(lit.) |
| alpha | 15.4 º (c=4, 6N HCl) |
| density | 1,293 g/cm3 |
| FEMA | 3297 |
| Fp | 145-148°C |
| storage temp. | Store at RT. |
| solubility | 1 M HCl: 50 mg/mL |
| form | powder |
| Water Solubility | 22.4 g/L (20 C) |
| Sublimation | 145-148 ºC |
| Merck | 14,5451 |
| BRN | 1721722 |
| Stability: | Stability Moisture and light sensitive. Incompatible with strong oxidising agents. |
| CAS DataBase Reference | 61-90-5(CAS DataBase Reference) |
| NIST Chemistry Reference | Leucine(61-90-5) |
| EPA Substance Registry System | L-Leucine(61-90-5) |
| Safety Information |
| Safety Statements | 24/25 |
| WGK Germany | 3 |
| RTECS | OH2850000 |
| HS Code | 29224995 |
| MSDS Information |
| Provider | Language |
|---|---|
| L-Leu | English |
| SigmaAldrich | English |
| ACROS | English |
| ALFA | English |
| L-Leucine Usage And Synthesis |
| One of the eight essential amino acids |
L- leucine is one of the eight essential amino acids, and belonging to the aliphatic amino acids within twenty kinds of proteins. L- leucine and L- isoleucine and L-valine are called three branched chain amino acids. L- leucineLeucine and D-leucine are enantiomers . It is a white shiny hexahedral crystal or white crystalline powder at room temperature , Odorless, slightly bitter . In the presence of hydrocarbons ,it is stable in the aqueous mineral acid. Per gram is dissolved in 40ml water and about 100ml acetic acid. Very slightly soluble in ethanol or ether, dissolved in formic acid, dilute hydrochloric acid, a solution of alkali hydroxides and a solution of carbonates. L- leucine can promote insulin secretion, can lower blood sugar; promote sleep, reduce sensitivity to pain, migraine headaches, anxiety and ease tension, reduce symptoms of chemical reactions disorder in the body caused by alcohol , and helps control alcoholism; it has a role in the treatment of dizziness , also can promote skin wound and bone healing, so doctors usually advise patients to take leucine supplements after surgery. amino acid infusion and comprehensive amino acid preparations are often formulated to be used in the treatment or health care; also be used as food, cosmetics and feed additives and plant growth promoter. The best food sources of leucine include brown rice, beans, meat, nuts, soy flour, and whole wheat. Excessive intake can also cause side effects, high intake can cause pellagra, vitamin A deficiency, dermatitis, diarrhea, mental disorders and other issues,which needs to be paid attention to . Diet containing too much leucine will increase the number of ammonia in the body and destroy the liver and kidney function. Therefore, patients with impaired liver or kidney function should not be excessive intake, otherwise it will exacerbate the condition. |
| Identification Test | Sublimation at about 150 ℃ . |
| Content Analysis |
Accurately weigh about 400mg sample after 2h of the pre-dried at 105 ℃ , move it into 250ml flask. Plus formic acid 3ml, 50ml glacial acetic acid to dissolve it. Add 2 drops of crystal violet test solution (TS-74) then use 0.1mol / L perchloric acid to titrate to a blue-green endpoint. At the same time, carry out a blank test and make the necessary corrections. 0.1mol / L perchloric acid is equivalent to L- leucine (C6 H13 NO2) 13.12mg per Ml. The above information is edited by the Chemicalbook of Tian Ye. |
| toxicity |
Safe for food (FDA, §172.3202000). LD505379mg / kg (rat, subcutaneously). |
| Application limitation | It accounts for6.4%of total protein in food (FDA§172.320,2000). |
| The maximum amount permitted of food additives maximum allowable residue limits |
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| Chemical Properties |
White shiny hexahedral crystals or white crystalline powder. Slightly bitter (DL- leucine is sweet). Sublimation at 145 ~ 148 ℃. Melting point 293 ~ 295 ℃ (decomposition). It belongs to the essential amino acids,and adult men requirement is 2.2g / d (151 version). It is necessary in normal growth for infants and maintaining normal nitrogen balance for adults. Natural products are found in the spleen, heart, etc., and are present in a variety of plant and animal tissues in the form of the proteins, free out after decomposition and corruption. |
| Application |
Amino acid drugs. Used as amino acid infusion and comprehensive amino acid preparations. For the diagnosis and treatment of children with idiopathic high blood sugar and glucose metabolism disorders,bile liver disease associated with reduced secretion , anemia, poisoning, muscular dystrophy, poliomyelitis, neuritis and mental illness. Diabetes, cerebral vascular sclerosis and kidney disease associated with proteinuria and hematuria is contraindicated. Gastric and duodenal ulcer patients should not be served. The product is used as a nutritional supplement,an amino acid infusion preparation and a comprehensive amino acid preparation,a hypoglycemic agent, a plant growth promoter.The product can be used as a spice according to GB 2760-8 . |
| Production Method |
with blood meal as a raw material BM [HCl (decomposition)] → [110 ℃, 24h] hydrolyzate [disacidify] → [vacuum distillation] disacidify fluid [activated charcoal (adsorption decolorization)] → effluent [concentrated] → [vacuum distillation] In addition to the concentrated solution [precipitation] → [o-xylene 4-sulfonic acid] precipitation [ammonia (free)] → [filter] leucine crude [activated charcoal (decolorizing)] → [70 ℃, 1h] filtrate [concentration crystallization] → [Save pressure distillation] L- leucine crude [water (washing and drying) → L- leucine finished Hydrolysis, disacidify add 500L 6mol / LHCl solution into a 1t Hydrolysis tank , and put in 100kg animal blood meal, heat insulation 110-120 ℃ reflux hydrolysis 24h, at 70-80 ℃ concentrate to a paste under reduced pressure. Add 50L and concentrate to a paste ,repeat three times, and cool to room temperature, hydrolyzate is collected by filtration . Adsorption, decolorization dilute 1-fold the hydrolyzate , at a 0.5L / min flow rate flow into the granular activated carbon column (30cm × 180cm), until the effluent appears phenylalanine, use deionized water to wash the solution at the same velocity till pH of 4.0, and combine the effluent and washings to get the effluent. Concentration, precipitation and free the effluent is concentrated 1/3 of its original volume under reduced pressure , add 1/10 volume (V / V) of 4-sulfonic acid o-xylene with stirring, produce leucine salt precipitation , filter the precipitate , wash with 2 volumes of deionized water stir, then add 6mol / L of ammonia to pH 6-8, stir at 70-80 ℃ incubated 1h, so leucine frees from sulfonate . Cooling and crystallization, crystals are collected by filtration, and then wash with 2 volumes of deionized water 2 times, get the crude leucine by suction pressure . Purification The crude productwas are dissolved in 40 volumes of deionized water , add 0.5% active carbon (5g / L) incubated 70 ℃, decolorizing stirring 1h, filtration of the filtrate, which is concentrated to 1/4 of its original volume, cooling and crystallization, crystals are collected by filtration, and washed with a small amount of water, drained 70-80 ℃ drying, get the L- leucine boutique . corn gluten as a raw material Corn gluten [dried] → corn gluten meal [alcohol extraction] → zein [hydrolysis] → hydrolyzate [neutralization, bleaching] → destaining solution [except tyrosine] → filtrate [106-110 ℃, 20h ; concentrated] → concentrate [acid-soluble] → precipitation [ammonolysis] → leucine crude [recrystallized] → L- leucine Preparation quality corn gluten meal corn gluten contains a lot of water, about 20% -30% solids, to be dehydrated and dried to obtain only corn gluten meal. Corn protein preparation to take corn gluten meal, with 90% -95% ethanol extract, recover ethanol extract is concentrated by evaporation, precipitated with water to obtain the zein. Hydrolysis take corn 10kg, industrial hydrochloric acid 27L, water 9L, put into hydrolysis tank, heat 106-110 ℃ 20h, hydrolyzate to reddish brown. Neutralization, bleach the hydrolyzate to cool with stirring, slowly add a solution of NaOH 7mol / L, and after neutralized to pH3, then add about 2kg activated carbon, stirred incubated 70-80 ℃, 30min decolorized, filter and the filtrate is taken destaining solution. So destaining solution is light yellow transparent solution. The decolorized solution is cooled to tyrosine and stirring constantly, add a small amount of tyrosine crystals, standing 24h, tyrosine crystals. The crystals are collected by suction filtration, crude tyrosine is obtained after washing , it is refined to get the products , reserving the mother liquor. Concentrating and crystallizing adjust the mother liquor described above with dilute HCl to pH 2.5, and then concentrate under reduced pressure, until there is a large number of crystallization of sodium chloride, the filtration, the filtrate is concentrated until the volume of 10-15L date, leaching, combine cake (leucine and sodium chloride mixed crystals). Adjuste filtrate with lye to pH3.3, stirring constantly, and add a small amount of the works of glutamate, glutamic acid is precipitated crystals of glutamic acid leaching is crude, refined to get the products. acidolysis acid soluble precipitate cake is combined (mixed crystals of sodium chloride and leucine), add 3mol / 7.5L L hydrochloric acid, the solution is heated and stirred, incubated at 70-80 ℃ for 0.5h, sodium chloride crystals is removed by sucking the filtratration of the solution of leucine hydrochloride, measuring about 13L, and secondly 10% by volume of o-xylene is added to the filtrated 4-sulfonic acid, leucine salts forms entirely precipitate is filtered off by filtration , and then filtrate in the same manner, and the filtrate is added 4-sulfonic acid o-xylene and then no precipitation so far. The filter cake is combined with a small amount of distilled water, stir, suction, wash 2 times ,give leucine salt crystals. Leucine free leucine salt crystals are added 7mol / L aqueous ammonia and the solution is neutralized to pH6-8, holding at 70-80 ℃ stirring for 1h, cooling and crystallization, filtrate to get the crystallization and wash with a little distilled water twice to give the crude leucine. Recrystallization weigh ratio of 1/40 with distilled water, leucine crude is heated to dissolve, then add 1% activated carbon bleaching. Destain solution chroma and clarity after passing inspection, concentrate under reduced pressure until a volume of up to 1/4 of the original volume, with stirring, cooling and crystallization, filtration is leucine crystals. Decolorizing concentrated mother liquor crystallizes, combine crystallization , and dry leucine is finished. horse-faced fish fillets Tun waste as raw material Horse-faced fish fillets Tuen waste [industrial hydrochloric acid (hydrolysis)] → hydrolyzate [and decolorization, 7mol / L NaOH] → [activated carbon; pH3] transparent liquid [dilute HCl (concentration crystallization)] → [pH2.5] cake [HCl, precipitating agent (acid-soluble precipitate)] → precipitate [7mol / L ammonia (ammonia solution)] → [pH6-8] crude [distilled water (recrystallized)] → L- leucine finished. Hydrolysis take horse-faced fish scraps 2kg, hydrochloric acid 5.4L, water 1.8L, hydrochloric acid concentration of industry about 6mol / L, heat insulation 106-110 ℃, hydrolysis 20h to reddish brown. Neutralization, bleaching the hydrolyzate is cooled with stirring, is added a solution of NaOH 7mol / L neutralized to bring the pH to 3. Then add about 2% of the hydrolyzate activated carbon, heat 70-80 ℃ stirring decolorization half an hour, filter and the filtrate, obtain a yellow transparent liquid. Concentrated, decolorized The yellow transparent liquid is adjusted to pH 2.5 with dilute HCl, concentrate under reduced pressure, until there is a lot of salt crystallization while, filter and filtrate the concentrated to a total volume of up to about 2-3L, filter, combine cake (NaCl and leucine mixed crystals). Acid-soluble, precipitate on the combined cake, HCl solution is added 1.5L 3mol / L, and heated and stirred, 70-80 ℃ incubated for half an hour, filtered off and NaCl crystals, filtrate the hydrochloride solution to obtain leucine . Dry hemoglobin as a raw material in sulfuric acid at reflux for several hours, soda neutralizes sedimentation, filtration, crystallization, with treatment of concentrated hydrochloric acid and 2-bromo-5-sodium toluene , hydrolysis ,wash with diethyl ether, crystallize to get the product. Hydrochloric acid or sulfuric acid hydrolysis of protein (casein, keratin, etc.) neutralized with alkali,to get leucine precipitation. This precipitate is mixed with L- isoleucine and L- methionine, with copper refining method, and then precipitate with β- naphthalene sulfonic acid to give L- leucine pure crystals. |
| Chemical Properties | White crystalline powder |
| L-Leucine Preparation Products And Raw materials |
| Preparation Products | L-Phenylalanine-->N-Acetyl-L-leucine-->L(+)-Leucinol-->N-BOC-L-leucylglycyl-arginine-p-nitroanilide hydrochloride |
| Raw materials | Hydrochloric acid-->Sulfuric acid -->Ammonia-->Ammonium bicarbonate-->Activated carbon-->Α-PROTEIN-->1,2-Dimethylbenzene-->Casein-->(2S,3S)-2-Amino-3-methylpentanoic acid-->L-Methionine-->Naphthalene-2-sulfonic acid -->HEMOGLOBIN-->2-Bromotoluene-->Phenprobamate-->N-CARBOBENZOXY-DL-LEUCINE-->KERATIN-->granular active carbon |